McGann T C, Kearney R D, Donnelly W J
J Dairy Res. 1979 Apr;46(2):307-11. doi: 10.1017/s0022029900017210.
Skim-milk was fractionated by permeation chromatography on CPG-10 (300 nm) at 20 degrees C with synthetic milk serum. The elution profile, which was highly reproducible, showed 3 partly resolved peaks. On treatment with 0.1% glutaraldehyde before chromatography, a similar profile was obtained indicating no appreciable micellar dissociation during chromatography of the milk. The casein composition of the eluent fractions, determined by quantitative chromatography on hydroxyapatite, showed that 12.1% of total casein was associated with the low molecular weight peak. The micelle content of beta- and kappa-casein increased with increasing elution volume, and the alphas/kappa ratio showed a progressive diminution from 4.3 to 2.3. Large differences were found in the colloidal phosphate contents and the weight average diameters of the micelle fractions which clearly show that CPG-10 fractionates on the basis of molecular size.
脱脂乳在20℃下用合成乳清在CPG - 10（300纳米）上通过渗透色谱法进行分级分离。洗脱图谱具有高度可重复性，显示出3个部分分离的峰。在色谱分离前用0.1%戊二醛处理后，得到了相似的图谱，表明在乳的色谱分离过程中没有明显的胶束解离。通过在羟基磷灰石上的定量色谱法测定洗脱级分的酪蛋白组成，结果表明总酪蛋白的12.1%与低分子量峰相关。β-酪蛋白和κ-酪蛋白的胶束含量随洗脱体积的增加而增加，αs/κ比值从4.3逐渐降低到2.3。在胶束级分的胶体磷酸盐含量和重均直径方面发现了很大差异，这清楚地表明CPG - 10是根据分子大小进行分级分离的。
