Schumacher M J, Pain M C
J Allergy Clin Immunol. 1979 Sep;64(3):202-8. doi: 10.1016/0091-6749(79)90096-4.
Nasal sensitivity to rye grass pollen allergens was evaluated by provocation testing in patients with hay fever due to grass pollen using measurements of nasal airways resistance (NAR), a reproducible system for delivery of allergen, and stringent criteria for allergen storage. Reproducibility was assessed in 24 subjects with hay fever by nasal provocation with serial dilutions of Lolium perenne allergens on 3 occasions: during the grass pollen season, immediately after the season, and in early winter. Threshold doses of allergen required to double the saline control NAR or to provoke persistent sneezing and rhinorrhea were slightly higher 1 mo after the pollen season, but there was no significant differences between threshold doses during the pollen season and 8 mo later. When the threshold doses during challenges were exceeded, there were late reactions in 4 of 24 patients. Normal subjects and patients with perennial rhinitis and with negative skin tests to L. perenne extract were unresponsive in nasal challenge tests.
通过激发试验，使用鼻气道阻力（NAR）测量、可重复的变应原递送系统以及严格的变应原储存标准，对因草花粉引起花粉症的患者进行黑麦草花粉变应原的鼻敏感性评估。通过在草花粉季节、季节刚结束后以及初冬这三个时间段，对24名花粉症患者进行黑麦草变应原系列稀释液的鼻激发试验，来评估再现性。使生理盐水对照NAR加倍或引发持续性打喷嚏和鼻溢所需的变应原阈值剂量在花粉季节结束后1个月略高，但花粉季节期间和8个月后的阈值剂量之间没有显著差异。当激发试验期间超过阈值剂量时，24名患者中有4名出现迟发反应。正常受试者以及对黑麦草提取物皮肤试验阴性的常年性鼻炎患者在鼻激发试验中无反应。
