Ortonne J P, Khatchadourian C, Voulot C, Menezo Y
J Invest Dermatol. 1979 Sep;73(3):239-42. doi: 10.1111/1523-1747.ep12514318.
Acetylcholinesterase (Ache) and pseudocholinesterase (BUche) activities were studied quantitatively in healthy skin by spectrophotometric methods and qualitatively by polyacrylamide gel electrophoresis. The results were compared to those obtained in plasma. The substrates used to reveal enzyme activities were acetylthiocholine (ATC) iodide and butyrylthiocholine (BTC) iodide, respectively. A linear relationship exists between the values of BUche and Ache activities in plasma and those in skin. Six isoenzymes of different electrophoretic mobility were observed in the skin. One of them, which is never found in plasma extracts, appears to be specific to the skin. On gradient gel electrophoresis, with both substrates (ATC and BTC), a single band of enzyme activity, corresponding to a molecular weight of 600,000 was observed. These results suggest that in the skin there is only one enzyme, most probably butyryl cholinesterase, which cleaves BTC somewhat faster than ATC. This methodology, when applied to the study of dermatoses in which abnormalities of cutaneous nerve terminals are suspected, should furnish precise functional pathophysiological details.
采用分光光度法对健康皮肤中的乙酰胆碱酯酶（Ache）和假性胆碱酯酶（BUche）活性进行了定量研究，并通过聚丙烯酰胺凝胶电泳进行了定性研究。将结果与血浆中的结果进行了比较。用于揭示酶活性的底物分别是碘化乙酰硫代胆碱（ATC）和碘化丁酰硫代胆碱（BTC）。血浆和皮肤中BUche和Ache活性值之间存在线性关系。在皮肤中观察到六种具有不同电泳迁移率的同工酶。其中一种在血浆提取物中从未发现，似乎是皮肤特有的。在梯度凝胶电泳中，使用两种底物（ATC和BTC）时，观察到一条对应分子量为600,000的单一酶活性带。这些结果表明，皮肤中只有一种酶，很可能是丁酰胆碱酯酶，它裂解BTC的速度比ATC略快。当将这种方法应用于怀疑存在皮肤神经末梢异常的皮肤病研究时，应该能够提供精确的功能病理生理学细节。
