Venkataraman B V, Rani M A, Andrade C, Joseph T
Department of Pharmacology, St. John's Medical College, Bangalore.
Indian J Physiol Pharmacol. 1993 Jan;37(1):82-4.
A modified colorimetric method for the estimation of cholinesterase activity has been worked out using two different substrates, acetylthiocholine iodide for total cholinesterase and a specific substrate, butyrylthiocholine iodide for pseudocholinesterase in the same sample. This is a modification of the method described by Voss and Sachsse (1970) wherein acetylthiocholine iodide was used for both total and pseudo cholinesterase activities. The pseudocholinesterase obtained with acetylthiocholine iodide was significantly higher (P < 0.0001) than that with butyrylthiocholine iodide either in whole blood or serum samples. Acetylthiocholine iodide while reacting with pseudocholinesterase in serum or plasma samples might also be interacting with the small quantities of acetylcholinesterase present. It is therefore suggested that butyrylthiocholine iodide and acetylthiocholine iodide may be used to determine pseudocholinesterase and total cholinesterase activities respectively. The use of two substrates with a few more alterations in the experimental conditions increased the validity of this simple and rapid colorimetric method.
已制定出一种改良的比色法来测定胆碱酯酶活性，该方法在同一样品中使用两种不同的底物，碘化乙酰硫代胆碱用于测定总胆碱酯酶，碘化丁酰硫代胆碱作为假性胆碱酯酶的特异性底物。这是对Voss和Sachsse（1970年）所描述方法的改良，他们在测定总胆碱酯酶活性和假性胆碱酯酶活性时均使用碘化乙酰硫代胆碱。无论是在全血还是血清样本中，用碘化乙酰硫代胆碱测得的假性胆碱酯酶活性均显著高于用碘化丁酰硫代胆碱测得的结果（P < 0.0001）。碘化乙酰硫代胆碱在与血清或血浆样本中的假性胆碱酯酶反应时，可能也会与其中存在的少量乙酰胆碱酯酶发生相互作用。因此建议分别使用碘化丁酰硫代胆碱和碘化乙酰硫代胆碱来测定假性胆碱酯酶活性和总胆碱酯酶活性。在实验条件上再做一些改变，使用这两种底物提高了这种简单快速比色法的有效性。
