细胞培养中视网膜色素细胞间缝隙连接和紧密连接的形成。

The formation of gap and tight junctions between retinal pigment cells in cell cultures.

作者信息

Meller K

出版信息

J Neurocytol. 1979 Apr;8(2):229-38. doi: 10.1007/BF01175563.

Abstract

The reformation of the junctional complex of retinal pigment cells was studied after trypsin disaggregation and in vitro reaggregation. Control specimens show a zonula occludens (tight junction) with integrated gap junctions and very large macular gap junctions. Isolation after trypsination results in disaggregation of the large gap junctions and fragmentation of the tight junctions with disaggregation of their integrated gap junctions. After two to four days of incubation the restoration of the zonula occludens is complete. After approximately five days of incubation, large gap junctions are found with a patchy arrangement of particles similar to that seen in vivo.

摘要

在胰蛋白酶消化和体外再聚集后，对视网膜色素细胞连接复合体的重塑进行了研究。对照标本显示有紧密连接（封闭小带），伴有整合的缝隙连接和非常大的黄斑缝隙连接。胰蛋白酶处理后的分离导致大缝隙连接解体，紧密连接碎片化，其整合的缝隙连接也解体。孵育两到四天后，紧密连接的恢复完成。孵育约五天后，发现大缝隙连接，其颗粒呈斑片状排列，类似于体内所见。