犬附睾中的类固醇受体。

Steroid receptors in the canine epididymis.

作者信息

Younes M, Evans B A, Chaisiri N, Valotaire Y, Pierrepoint C G

出版信息

J Reprod Fertil. 1979 May;56(1):45-52. doi: 10.1530/jrf.0.0560045.

DOI:10.1530/jrf.0.0560045

Abstract

High-affinity and low-capacity molecules that bind 5 alpha-dihydrotestosterone and oestradiol-17 beta have been demonstrated in the canine epididymis. The molecules were shown to be proteinaceous in nature, thermolabile and sedimented in a sucrose density gradient with a coefficient of 8S with respect to bovine serum albumin. Only minor differences were found for dissociation constants for oestradio-17 beta (3.5 +/- 1.74 S 10(-10) M) and 5 alpha-dihydrotestosterone (4.2 +/- 10.75 X 10(-10) M) (means +/- s.d.) in the three anatomically distinct regions of the epididymis.

摘要

在犬附睾中已证实存在能结合5α-双氢睾酮和雌二醇-17β的高亲和力、低容量分子。这些分子本质上为蛋白质，不耐热，在蔗糖密度梯度中沉降，相对于牛血清白蛋白的沉降系数为8S。在附睾三个解剖学上不同的区域，雌二醇-17β（3.5±1.74×10⁻¹⁰M）和5α-双氢睾酮（4.2±10.75×10⁻¹⁰M）（平均值±标准差）的解离常数仅存在微小差异。