Shirley M W
Parasitology. 1979 Apr;78(2):221-37. doi: 10.1017/s003118200004926x.
An examination of 2 strains of Eimeria acervulina var. mivati (since 1973 E. mivati has been regarded as a variant of E. acervulina) showed that previous confusion over the taxonomic status of E. mivati arose because the investigations were done using laboratory cultures of E. mivati which were contaiminated with E. acervulina. Electrophoretic analyses of enzymes, host specificity and cross-immunity tests have revealed that: (1) The 1971 Houghton strain of E. acervulina var. mivati was a mixture of 2 parasites. (a) Passage of this strain in embryonating eggs resulted in a selection against that parasite previously characterized as E. acervulina. (b) The parasite which did reproduce in eggs did not immunize chickens against subsequent challenge with E. acervulina. This parasite is most likely E. mivati. (c) E. mivati recovered from eggs did, however, immunize chickens against challenge with a new field strain which was morphologically identical to E. mivati and characterized by the same electrophoretic forms of 2 enzymes. (2) A strain of E. acervulina var. mivati from the USA was also a mixture of E. acervulina and E. mivati.
对2株堆型艾美耳球虫米瓦蒂变种（自1973年以来，米瓦蒂艾美耳球虫一直被视为堆型艾美耳球虫的一个变种）的研究表明，先前对米瓦蒂艾美耳球虫分类地位的混淆是由于使用受堆型艾美耳球虫污染的米瓦蒂艾美耳球虫实验室培养物进行研究造成的。酶的电泳分析、宿主特异性和交叉免疫试验表明：（1）1971年霍顿株堆型艾美耳球虫米瓦蒂变种是2种寄生虫的混合物。（a） 该株在胚胎化卵中传代导致对先前被鉴定为堆型艾美耳球虫的寄生虫进行了选择。（b） 在卵中繁殖的寄生虫不能使鸡对随后的堆型艾美耳球虫攻击产生免疫。这种寄生虫很可能是米瓦蒂艾美耳球虫。（c） 然而，从卵中回收的米瓦蒂艾美耳球虫确实能使鸡对一种新的田间株攻击产生免疫，该田间株在形态上与米瓦蒂艾美耳球虫相同，并以2种酶的相同电泳形式为特征。（2） 来自美国的一株堆型艾美耳球虫米瓦蒂变种也是堆型艾美耳球虫和米瓦蒂艾美耳球虫的混合物。
