Shirley M W
Z Parasitenkd. 1978 Sep 4;57(1):83-7. doi: 10.1007/BF00927630.
Embryo-adapted strains of Eimeria mivati and E. mivati var. diminuta, differing in their sensitivity to sulphaquinoxaline and electrophoretic mobilities of lactate dehydrogenase, were crossed. E. mivati was sulphaquinoxaline-resistant and characterised by an electrophoretic form of the enzyme denoted lactate dehydrogenase-1 whereas E. mivati var. diminuta was sulphaquinoxaline-sensitive and characterised by lactate dehydrogenase-6. Progeny recovered from the cross were passaged in embryonating eggs given sulphaquinoxaline and the (drug-resistant) parasites recovered were characterised by both lactate dehydrogenase-1 and lactate dehydrogenase-6. Controls showed that those parasites characterised by the recombinant phenotype of drug-resistant and lactate dehydrogenase-6 had been produced by the cross-fertilisation of gametes.
对磺胺喹恶啉的敏感性以及乳酸脱氢酶电泳迁移率存在差异的米氏艾美耳球虫胚胎适应株和微小米氏艾美耳球虫变种进行了杂交。米氏艾美耳球虫对磺胺喹恶啉具有抗性，其特征在于该酶的一种电泳形式，即乳酸脱氢酶 -1，而微小米氏艾美耳球虫变种对磺胺喹恶啉敏感，其特征在于乳酸脱氢酶 -6。从杂交后代中回收的子代在添加了磺胺喹恶啉的胚胎化卵中传代培养，回收的（耐药）寄生虫同时具有乳酸脱氢酶 -1 和乳酸脱氢酶 -6 的特征。对照表明，那些具有耐药和乳酸脱氢酶 -6 重组表型特征的寄生虫是由配子杂交受精产生的。
