Cooper A G, Brown M C, Derby H A, Wortis H H
Clin Exp Immunol. 1973 Apr;13(4):487-96.
Using automated haemagglutination and haemagglutination-inhibition methods which are capable of measuring gamma, mu or kappa chains in the nanogram range, we have examined a variety of normal and neoplastic human lymphocyte populations for the amounts of their surface-membrane and internal immunoglobulins. We find IgG to be the predominant immunoglobulin on the surface-membrane and within various normal human lymphocytes, including thymocytes. IgM, in addition to IgG, is found on the surface of tonsil cells. Chronic lymphatic leukaemic lymphocytes consistently had small amounts of surface-membrane immunoglobulin and larger amounts internally (usually IgM), whereas large amounts of immunoglobulin (usually IgM) were present on the surface-membranes and internally in leukaemic lymphosarcoma cells. Both types of cells had the B-specific surface-antigens. Different types of lymphatic leukaemias may represent neoplastic counterparts of different stages of B cell differentiation.
利用能够在纳克范围内测量γ、μ或κ链的自动血凝和血凝抑制方法，我们检测了各种正常和肿瘤性人类淋巴细胞群体的表面膜和内部免疫球蛋白含量。我们发现IgG是包括胸腺细胞在内的各种正常人类淋巴细胞表面膜上以及细胞内的主要免疫球蛋白。除IgG外，IgM存在于扁桃体细胞表面。慢性淋巴细胞白血病淋巴细胞表面膜免疫球蛋白含量始终较少，而细胞内含量较多（通常为IgM），而白血病性淋巴肉瘤细胞表面膜和细胞内均存在大量免疫球蛋白（通常为IgM）。这两种细胞都有B特异性表面抗原。不同类型的淋巴细胞白血病可能代表B细胞分化不同阶段的肿瘤对应物。
