Johnson B F, Yoo B Y, Calleja G B
J Bacteriol. 1973 Jul;115(1):358-66. doi: 10.1128/jb.115.1.358-366.1973.
Electron microscopy of dividing fission yeast cells shows establishment of an annular rudiment (AR) of electron-transparent material under the old cell wall as the first sign of elaboration of the cell plate. The AR grows centripetally, finally closing at the mid-point of the cell. During the inward growth of the AR it is thickened by addition of denser material which becomes the scar plug after fission; the electron-transparent material is lost at fission. Lying always between the cytoplasmic membrane and the cell wall is a dark layer of variable thickness. This layer becomes markedly thickened into a fillet at the base of the centripetally growing cell plate. The fission process begins after the cell plate is completely elaborated. One striking feature of fission is the migration of dense material from the fillet at the base of the cell plate outwardly through the matrix of the cell wall to its final resting place as a dark ring, a "fuscannel," adjacent to the fission scar. The inclusion of Golgi bodies in many sections suggests their involvement in cell plate elaboration, presumably through production of the dense bodies which are seen to fuse with the dark layer proximal to the growing cell plate.
对正在分裂的裂殖酵母细胞进行电子显微镜观察发现，在旧细胞壁下方会形成一个由电子透明物质构成的环状雏形（AR），这是细胞板形成的首个迹象。AR向心生长，最终在细胞中点处闭合。在AR向内生长的过程中，会因添加更致密的物质而增厚，这些物质在分裂后成为疤痕栓；电子透明物质在分裂时消失。在细胞质膜和细胞壁之间始终存在一层厚度可变的深色层。在向心生长的细胞板基部，这一层会明显增厚形成一条带状物。细胞板完全形成后，分裂过程开始。分裂的一个显著特征是，致密物质从细胞板基部的带状物向外穿过细胞壁基质，迁移至其最终停留位置，形成一个与分裂疤痕相邻的深色环，即“fuscannel”。许多切片中都包含高尔基体，这表明它们参与了细胞板的形成，推测是通过产生致密体来实现的，这些致密体可与生长中的细胞板近端的深色层融合。
