Baar S
Br J Exp Pathol. 1973 Jun;54(3):322-8.
A study of glycolysis of red cells heated in their own plasma for 10 min at 48° has shown that heat treatment alone increased total glucose utilization of AB cells by 12%, A cells by 11%, B cells by 9·3% and O cells by 11·9%. Subsequent incubation for 3 hours at 37° further increased glycolysis by an additional 88% for AB cells, 39% for A cells, 68% for B cells and 41% for O cells, indicating that the rate of increased glucose utilization of the cells examined was quantitatively related to the degree of initial stimulation during heating. Aerobic glycolysis increased by 40% to 55% in AB, A and O cells during heating; B cells showed only a 10% increase. Incubation at 37° following heating reversed the heat stimulus. AB and A cells now showed a decrease of 33% and 36% while both B and O cells only showed a reduction of 17%. Lactate/glucose ratios are lowered both after heating and after subsequent incubation at 37°. ATP levels were reduced between 4% and 9% by heating at 48°. The greatest reduction occurred in the A cells, which also showed the greatest increase in glycolysis during this period. Subsequent incubation increased the ATP deficit to about 14% for B and O cells, 7% for AB cells but 39% for A cells. The possible implications of the findings are discussed.
一项关于红细胞在自身血浆中于48°加热10分钟后糖酵解的研究表明，仅热处理就使AB型细胞的总葡萄糖利用率提高了12%，A型细胞提高了11%，B型细胞提高了9.3%，O型细胞提高了11.9%。随后在37°孵育3小时，AB型细胞的糖酵解进一步增加了88%，A型细胞增加了39%，B型细胞增加了68%，O型细胞增加了41%，这表明所检测细胞的葡萄糖利用率增加速率与加热期间的初始刺激程度在数量上相关。在加热过程中，AB型、A型和O型细胞的有氧糖酵解增加了40%至55%；B型细胞仅增加了10%。加热后在37°孵育逆转了热刺激。AB型和A型细胞现在分别下降了33%和36%，而B型和O型细胞仅下降了17%。加热后以及随后在37°孵育后，乳酸/葡萄糖比率均降低。在48°加热使ATP水平降低了4%至9%。最大的降低发生在A型细胞中，在此期间其糖酵解也增加最多。随后的孵育使B型和O型细胞的ATP亏缺增加到约14%，AB型细胞增加到7%，而A型细胞增加到39%。讨论了这些发现的可能意义。
