体外实验性肉芽组织的氨基酸代谢
Amino acid metabolism of experimental granulation tissue in vitro.
作者信息
Aalto M, Lampiaho K, Pikkarainen J, Kulonen E
出版信息
Biochem J. 1973 Apr;132(4):663-71. doi: 10.1042/bj1320663.
Abstract
- The intracellular volume in granulation tissue was about 15% of the total urea space. 2. The experimental granuloma has a greater ability to retain amino acids during the proliferation phase than later during the synthesis of collagen. 3. The synthesis of collagen and other proteins by granulation tissue is related to the concentrations of proline and glutamic acid in the medium. 4. The rate of synthesis of proline from glutamic acid in granulation-tissue slices is greatest during collagen synthesis. It is enhanced by lactate. 5. Extracellular cations influence the synthesis of collagen and ouabain is inhibitory. Synthesis of other proteins is less sensitive in this respect. 6. It is suggested that the synthesis of collagen is related to the supply of certain amino acids, especially proline, and hence to the redox balance, and also to the function of the cell wall.
摘要
- 肉芽组织中的细胞内体积约占总尿素空间的15%。2. 实验性肉芽肿在增殖期比在后期胶原合成阶段保留氨基酸的能力更强。3. 肉芽组织中胶原和其他蛋白质的合成与培养基中脯氨酸和谷氨酸的浓度有关。4. 肉芽组织切片中从谷氨酸合成脯氨酸的速率在胶原合成期间最大。乳酸可增强该速率。5. 细胞外阳离子影响胶原的合成，哇巴因具有抑制作用。在这方面，其他蛋白质的合成不太敏感。6. 有人提出，胶原的合成与某些氨基酸的供应有关，尤其是脯氨酸，因此与氧化还原平衡有关，也与细胞壁的功能有关。
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