来自角膜和骺板软骨的尿苷二磷酸葡萄糖脱氢酶。
Uridine diphosphate glucose dehydrogenase from cornea and epiphysial-plate cartilage.
作者信息
Balduini C, Brovelli A, De Luca G, Galligani L, Castellani A A
出版信息
Biochem J. 1973 Jun;133(2):243-9. doi: 10.1042/bj1330243.
Abstract
- UDP-glucose dehydrogenase (EC 1.1.1.22) was extracted from epiphysial-plate cartilage of newborn pigs and from whole bovine corneas. 2. Formation of UDP-glucuronic acid was demonstrated by radioautography after separation of the sugar nucleotides by paper chromatography or t.l.c.: in these conditions a radioactive glucuronic acid spot also appears. 3. UDP-xylose prevented the formation in the incubation mixture of both UDP-glucuronic acid and free glucuronic acid. 4. In both tissues the dependence of the enzyme activity on pH and the K(m) values for UDP-glucose and NAD(+) were determined. 5. Inhibition by UDP-xylose with respect to UDP-glucose was investigated. The plots of 1/v versus 1/[UDP-glucose], and of percentage inhibition versus UDP-xylose concentration and the Hill coefficient showed that a co-operative effect existed between UDP-xylose-binding sites. 6. The physiological meaning of the different affinities of cartilage and cornea enzymes for UDP-xylose is discussed and related to the different glycosaminoglycan contents of the two connective tissues studied.
摘要
- UDP-葡萄糖脱氢酶（EC 1.1.1.22）从新生猪的骺板软骨和完整的牛角膜中提取。2. 通过纸色谱法或薄层层析法分离糖核苷酸后，经放射自显影证明了UDP-葡萄糖醛酸的形成：在这些条件下也出现了放射性葡萄糖醛酸斑点。3. UDP-木糖可防止孵育混合物中UDP-葡萄糖醛酸和游离葡萄糖醛酸的形成。4. 在两种组织中均测定了酶活性对pH的依赖性以及对UDP-葡萄糖和NAD(+)的K(m)值。5. 研究了UDP-木糖对UDP-葡萄糖的抑制作用。1/v对1/[UDP-葡萄糖]的曲线、抑制百分比对UDP-木糖浓度的曲线以及希尔系数表明UDP-木糖结合位点之间存在协同效应。6. 讨论了软骨和角膜酶对UDP-木糖不同亲和力的生理意义，并将其与所研究的两种结缔组织中不同的糖胺聚糖含量相关联。
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