Maheswaran S K, Thies E S
Res Vet Sci. 1979 Jan;26(1):25-31.
A whole blood lymphocyte stimulation assay to study cell-mediated immune responses in bovine pasteurellosis was developed. Peripheral blood lymphocytes from cattle artificially immunised with three Asiatic haemorrhagic septicaemia strains of Pasteurella multocida exhibited higher stimulation indices when incubated with antigen preparations from homologous strains than with the heterologous shipping fever strain. Lymphocytes from cattle immunised with the shipping fever strain of P multocida exhibited a higher stimulation index when incubated with an antigen preparation from the homolgous strain than with antigen preparations from heterologous haemorrhagic septicaemia strains. These results suggest that immunogenic differences exist between the haemorrhagic septicaemia strains and the shipping fever strain of P multocida. An assay using turkey whole blood lymphocytes was also developed. The use of small amounts of whole blood, microtitre plates, either 125I iododeoxyuridine or 3H-thymidine as the labelling agent, and a multiple cell-culture harvester makes the method simple, rapid and suitable for the study of immune competence and cell-mediated immune responses in turkeys on a flock basis.
开发了一种全血淋巴细胞刺激试验，用于研究牛巴氏杆菌病中的细胞介导免疫反应。用三种多杀性巴氏杆菌亚洲出血性败血症菌株人工免疫的牛的外周血淋巴细胞，与同源菌株的抗原制剂孵育时，其刺激指数高于与异源运输热菌株孵育时。用多杀性巴氏杆菌运输热菌株免疫的牛的淋巴细胞，与同源菌株的抗原制剂孵育时，其刺激指数高于与异源出血性败血症菌株的抗原制剂孵育时。这些结果表明，多杀性巴氏杆菌的出血性败血症菌株和运输热菌株之间存在免疫原性差异。还开发了一种使用火鸡全血淋巴细胞的试验。使用少量全血、微量滴定板、125I碘脱氧尿苷或3H-胸腺嘧啶作为标记剂，以及多细胞培养收获器，使该方法简单、快速，适用于在群体基础上研究火鸡的免疫能力和细胞介导免疫反应。
