家蚕基因组中丝素基因的长度。

The length of the fibroin gene in the Bombyx mori genome.

作者信息

Lizardi P M, Brown D D

出版信息

Cell. 1975 Mar;4(3):207-15. doi: 10.1016/0092-8674(75)90169-5.

Abstract

Radioactive anti-messenger DNA (3H-cDNA) complementary to silk fibroin mRNA has been synthesized using reverse transcriptase. This 3H-cDNA has been found to be a specific and sensitive probe for the detection of fibroin genes in the genome of Brombyx mori. Actinomycin-CsCl gradients give a large separation of the high GC fibroin genes from the bulk DNA. This density shift of fibroin genes has been measured as a function of DNA molecular weight. The data support a model in which a single high GC fibroin gene of 11.6 times 10-6 daltons is surrounded by at least 6 times 10-7 daltons of low GC DNA (30-39 percent). This finding, along with saturation hybridization studies (Suzuki, Gage, and Brown, 1972), demonstrate that the fibroin gene is present in a single copy per haploid genome.

摘要

已使用逆转录酶合成了与丝心蛋白信使核糖核酸（mRNA）互补的放射性抗信使脱氧核糖核酸（3H-cDNA）。已发现这种3H-cDNA是检测家蚕基因组中丝心蛋白基因的一种特异且灵敏的探针。放线菌素-CsCl梯度能使高GC含量的丝心蛋白基因与大量DNA产生较大程度的分离。已测定了丝心蛋白基因这种密度变化与DNA分子量的函数关系。这些数据支持这样一个模型：一个分子量为11.6×10⁻⁶道尔顿的单一高GC含量丝心蛋白基因被至少6×10⁻⁷道尔顿的低GC含量DNA（30%-39%）所包围。这一发现，连同饱和杂交研究（铃木、盖奇和布朗，1972年）表明，丝心蛋白基因在每个单倍体基因组中以单拷贝形式存在。