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人小肠刷状缘的分离、部分特性鉴定及亚分级分离

The isolation, partial characterization, and subfractionation of human intestinal brush borders.

作者信息

Houghton S E, McCarthy C F

出版信息

Gut. 1973 Jul;14(7):529-34. doi: 10.1136/gut.14.7.529.

DOI:10.1136/gut.14.7.529
PMID:4729919
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1412796/
Abstract

Brush borders were prepared from small intestinal specimens obtained from 20 patients who underwent abdominal surgery. Some enzyme constituents of the brush borders and fractions obtained from them by papain digestion are described.

摘要

从20名接受腹部手术的患者的小肠标本中制备刷状缘。本文描述了刷状缘的一些酶成分以及通过木瓜蛋白酶消化从刷状缘获得的组分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f35f/1412796/a51592c96901/gut00632-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f35f/1412796/a51592c96901/gut00632-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f35f/1412796/a51592c96901/gut00632-0029-a.jpg

相似文献

1
The isolation, partial characterization, and subfractionation of human intestinal brush borders.人小肠刷状缘的分离、部分特性鉴定及亚分级分离
Gut. 1973 Jul;14(7):529-34. doi: 10.1136/gut.14.7.529.
2
Enzymic solubilization of the human intestinal brush border membrane enzymes.人肠刷状缘膜酶的酶促溶解
Biochim Biophys Acta. 1976 May 21;433(3):469-81. doi: 10.1016/0005-2736(76)90274-1.
3
Regional variations in the composition of purified brush borders isolated from infant and adult rabbit small intestine.
Arch Int Physiol Biochim. 1971 Apr;79(2):279-86. doi: 10.3109/13813457109085309.
4
Solubilization of brush borders of hamster small intestine and fractionation of some of the components.仓鼠小肠刷状缘的增溶及部分成分的分级分离。
Biochim Biophys Acta. 1975 Jul 3;394(3):361-76. doi: 10.1016/0005-2736(75)90290-4.
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Quantitative distribution of some enzymes along the villi and crypts of human small intestine.人体小肠绒毛和隐窝中某些酶的定量分布。
Scand J Gastroenterol. 1973;8(5):406-16.
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Purification of the human intestinal brush border membrane.人小肠刷状缘膜的纯化
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Fine structural localization of non-specific alkaline phosphatase activity in the small intestine of the rat; an intravital perfusion experiment with azo dye method.大鼠小肠中非特异性碱性磷酸酶活性的精细结构定位；偶氮染料法活体灌注实验
Okajimas Folia Anat Jpn. 1971 Dec;48(5):323-31. doi: 10.2535/ofaj1936.48.5_323.
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Fractions of the brush border.刷状缘的各部分。
Fed Proc. 1969 Jan-Feb;28(1):30-4.
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Release of intestinal surface-membrane glycoproteins associated with enzyme activity by brief digestion with papain.通过木瓜蛋白酶短暂消化释放与酶活性相关的肠表面膜糖蛋白。
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Effect of vitamin D on brush border alkaline phosphatase in the rat small intestine.
Enzyme. 1972;13(5-6):278-85. doi: 10.1159/000459675.

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Infect Immun. 1980 Jun;28(3):1019-27. doi: 10.1128/iai.28.3.1019-1027.1980.
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[Old and new data on diarrhetic diseases in childhood I. Etiology and pathophysiology].[儿童腹泻病的新旧数据 I. 病因学与病理生理学]
Infection. 1975;3(3):127-42. doi: 10.1007/BF01641335.
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Characterization of bile acid binding to rat intestinal brush border membranes.

本文引用的文献

1
The intestinal brush border.肠刷状缘
Gut. 1971 Aug;12(8):668-77. doi: 10.1136/gut.12.8.668.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
The digestive function of the epithelium of the small intestine. II. Localization of disaccharide hydrolysis in the isolated brush border portion of intestinal epithelial cells.小肠上皮的消化功能。II. 二糖水解在肠上皮细胞分离刷状缘部分的定位。
J Membr Biol. 1977 May 12;33(3-4):213-30. doi: 10.1007/BF01869517.
Biochim Biophys Acta. 1961 Sep 16;52:293-8. doi: 10.1016/0006-3002(61)90678-3.
4
THE ESTIMATION OF DEOXYRIBONUCLEIC ACID IN THE PRESENCE OF SIALIC ACID: APPLICATION TO ANALYSIS OF HUMAN GASTRIC WASHINGS.在唾液酸存在的情况下对脱氧核糖核酸的估计：在人胃冲洗液分析中的应用。
Biochem J. 1965 Jun;95(3):612-20. doi: 10.1042/bj0950612.
5
INTESTINAL GLYCOSIDASE ACTIVITIES IN THE HUMAN EMBRYO, FETUS, AND NEWBORN.人类胚胎、胎儿及新生儿的肠道糖苷酶活性
Pediatrics. 1965 Jun;35:944-54.
6
A procedure for the isolation of the epithelial brush border membrane of hamster small intestine.一种分离仓鼠小肠上皮刷状缘膜的方法。
Anal Biochem. 1961 Jun;2:284-6. doi: 10.1016/s0003-2697(61)80014-6.
7
Intrinsic factor-mediated attachment of vitamin B12 to brush borders and microvillous membranes of hamster intestine.内因子介导维生素B12与仓鼠肠道刷状缘和微绒毛膜的附着。
J Clin Invest. 1967 Jul;46(7):1215-28. doi: 10.1172/JCI105615.
8
Disaccharidase: localization in hamster intestine brush borders.双糖酶：在仓鼠肠道刷状缘中的定位。
Science. 1967 Mar 31;155(3770):1670-2. doi: 10.1126/science.155.3770.1670.
9
Surface sugar in the intestine.肠道中的表面糖。
Am J Med Sci. 1969 Sep;258(3):172-80. doi: 10.1097/00000441-196909000-00004.
10
The intestinal brush border as a digestive and absorptive surface.
Am J Med Sci. 1969 Sep;258(3):144-9. doi: 10.1097/00000441-196909000-00002.