甘油对人体脂肪组织甘油三酯脂肪酶活性的影响。
Effects of glycerol on human adipose tissue triglyceride lipase activity.
作者信息
Giudicelli H, Boyer J
出版信息
J Lipid Res. 1973 Sep;14(5):592-5.
PMID:4729976
Abstract
Glycerol fully protects the human adipose tissue triglyceride lipase against the denaturing effects of high and low temperatures. Under such protection, storage of crude preparations at -10 degrees C or incubation at 50 degrees C resulted in a 1.5-3-fold increase of the measured lipase activity. This increase was shown to be related to enzyme newly released from tissular microparticles present in the samples. Advantage may be taken of these observations to improve greatly the conditions of extraction and storage of this lipase activity.
摘要
甘油能充分保护人脂肪组织甘油三酯脂肪酶免受高温和低温的变性影响。在这种保护下，粗制品在-10℃储存或在50℃孵育会使测得的脂肪酶活性增加1.5至3倍。这种增加表明与样品中存在的组织微粒新释放的酶有关。利用这些观察结果可大大改善这种脂肪酶活性的提取和储存条件。
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