Ianishpol'skiĭ V V, Tertykh V A, Liubinskiĭ G V
Ukr Biokhim Zh (1978). 1979 Jul-Aug;51(4):324-9.
The methods are suggested for immobilizing trypsin on the organo-silica surface by means of cyanuric chloride and maleic anhydride. The analysis of kinetics of the enzyme carrier coupling shows that at 22 degrees C the immobilization time on the surface of organo-silicas activated with cyanuric chloride and maleic anhydride is 70 and 35 min, respectively. The immobilized trypsin is shown to be more thermostable as compared to the soluble one. The half-life of the immobilized trypsin at 55 degrees C is about 40 h.
提出了通过三聚氯氰和马来酸酐将胰蛋白酶固定在有机硅表面的方法。对酶与载体偶联动力学的分析表明，在22℃时，用三聚氯氰和马来酸酐活化的有机硅表面上的固定时间分别为70分钟和35分钟。结果表明，与可溶性胰蛋白酶相比，固定化胰蛋白酶具有更高的热稳定性。固定化胰蛋白酶在55℃下的半衰期约为40小时。
