Suk'ina E G, Golod M G
Ukr Biokhim Zh (1978). 1979 Jul-Aug;51(4):400-3.
The article deals with the possibility of chlorophyll and chlorophyllase immobilization on methylaerosil and also with the effect of this carrier on the enzyme-substrate interaction. Chlorophyll and chlorophyllase are shown to be able to hydrophorbic interaction. It is established that the ultimate saturation of methylaerosil with chlorophyll is 16.1 mg per 100 mg of the carrier. The chlorophyllase reaction is the most active under conditions when the enzyme and substrate are in soluble states. The hydropholic binding of chlorophyll with methylaerosil decreases the intensity of its hydrolysis for the period of reaction and the preliminary immobilization of chlorophyllase on methylaerosil inhibits the reaction to a still greater extent. This evidences for the significance of the reaction system organization for interaction of chlorophyllase with chlorophyll.
本文探讨了叶绿素和叶绿素酶固定在甲基硅石上的可能性，以及这种载体对酶-底物相互作用的影响。研究表明，叶绿素和叶绿素酶能够发生疏水相互作用。已确定甲基硅石对叶绿素的最终饱和吸附量为每100毫克载体吸附16.1毫克叶绿素。当酶和底物处于可溶状态时，叶绿素酶反应最为活跃。叶绿素与甲基硅石的疏水结合在反应期间降低了其水解强度，而叶绿素酶预先固定在甲基硅石上则在更大程度上抑制了反应。这证明了反应体系组织对于叶绿素酶与叶绿素相互作用的重要性。
