Bystryn J C, Siskind G W, Uhr J W
J Exp Med. 1973 Feb 1;137(2):301-16. doi: 10.1084/jem.137.2.301.
The binding of antigen to cells with antibody on their surface has been studied in a model system consisting of murine myeloma cells (MOPC 315) and DNP conjugates. Specific binding occurred between the DNP groups of DNP conjugates and cell surface immunoglobulin. Using this model, the binding affinities of multivalent and univalent DNP conjugates were measured directly by equilibrium-binding techniques and indirectly by displacement of bound conjugate with univalent hapten. With both approaches the multivalent conjugate was shown to bind to cells with an avidity 100-300 fold greater than the univalent hapten. Nonspecific binding of unrelated protein and repeated washing of cells was found to markedly dedecrease the specific binding of univalent conjugates, presumably because the relatively weak bonds dissociate readily.
在一个由鼠骨髓瘤细胞（MOPC 315）和二硝基苯（DNP）偶联物组成的模型系统中，研究了抗原与表面带有抗体的细胞之间的结合。DNP偶联物的DNP基团与细胞表面免疫球蛋白之间发生了特异性结合。利用该模型，通过平衡结合技术直接测量了多价和单价DNP偶联物的结合亲和力，并通过单价半抗原置换结合的偶联物间接进行了测量。两种方法均表明，多价偶联物与细胞的结合亲和力比单价半抗原高100 - 300倍。发现无关蛋白质的非特异性结合以及对细胞的反复洗涤会显著降低单价偶联物的特异性结合，这可能是因为相对较弱的键容易解离。
