细胞黏附研究。3. 幼仓鼠肾细胞的黏附
Studies on cell adhesion. 3. Adhesion of baby hamster kidney cells.
作者信息
Grinnell F, Milam M, Srere P A
出版信息
J Cell Biol. 1973 Mar;56(3):659-65. doi: 10.1083/jcb.56.3.659.
Abstract
Normal and transformed baby hamster kidney (BHK) cells attach to Falcon polystyrene with the same first order rate constant. The longer the cells are attached to the bottles, the more difficult they are to remove. Sulfhydryl (-SH) binding reagents inhibit both the attachment of BHK cells and the increase in adhesive strength of attached cells. Attached BHK cells bind fewer molecules of [1-(14)C]N-ethylamleimide (an -SH binding reagent) than do suspended cells. Incubation of cells with high concentrations of trypsin results in a reversible loss of cell adhesiveness. The recovery of adhesiveness of trypsin-treated cells is inhibited by cycloheximide.
摘要
正常和转化的幼仓鼠肾（BHK）细胞以相同的一级速率常数附着于Falcon聚苯乙烯。细胞附着在瓶上的时间越长，就越难去除。巯基（-SH）结合试剂既抑制BHK细胞的附着，也抑制附着细胞黏附强度的增加。附着的BHK细胞比悬浮细胞结合的[1-(14)C]N-乙基马来酰亚胺（一种-SH结合试剂）分子更少。用高浓度胰蛋白酶孵育细胞会导致细胞黏附性的可逆丧失。环己酰亚胺抑制胰蛋白酶处理过的细胞黏附性的恢复。
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