Wilson B L, Mortlock R P
J Bacteriol. 1973 Mar;113(3):1404-11. doi: 10.1128/jb.113.3.1404-1411.1973.
Aerobacter aerogenes strain PRL-R3 possesses inducible enzyme pathways for the catabolism of d-xylose and d-arabitol. d-Xylose is the apparent inducer for d-xylose isomerase and d-xylulokinase. d-Arabitol is the apparent inducer for d-arabitol dehydrogenase and a separate d-xylulokinase. Both kinases had similar K(m) values and substrate specificities, and could not be separated by sucrose gradient centrifugation or polyacrylamide gel electrophoresis. They could be differentiated, however, by their separate regulation, their inhibition by antisera, and by the cold sensitivity of the kinase of the d-arabitol catabolic pathway.
产气气杆菌菌株PRL-R3拥有用于d-木糖和d-阿拉伯糖醇分解代谢的诱导酶途径。d-木糖是d-木糖异构酶和d-木酮糖激酶的明显诱导剂。d-阿拉伯糖醇是d-阿拉伯糖醇脱氢酶和一种单独的d-木酮糖激酶的明显诱导剂。两种激酶具有相似的K(m)值和底物特异性,并且不能通过蔗糖梯度离心或聚丙烯酰胺凝胶电泳分离。然而,它们可以通过各自的调节、抗血清对它们的抑制以及d-阿拉伯糖醇分解代谢途径中激酶的冷敏感性来区分。