Berman J J, Rice J M
Vet Pathol. 1979 Sep;16(5):574-82. doi: 10.1177/030098587901600510.
A single intraperitoneal dose of methyl(acetoxymethyl)nitrosamine (13 mg/kg body weight) given to 78 5-week-old male rats induced 25 mesotheliomas; two mesotheliomas were found in 67 control rats. All mesotheliomas arose from the peritesticular mesothelium and had a typical microscopic appearance of branching papillary fronds with a collagenous core covered by one or many layers of plump tumor cells. Cytoplasm of tumor cells contained material that reacted positively to a colloidal iron stain and was labile to hyaluronidase. In addition to frank mesotheliomas, 16 lesions, which we called atypical mesothelial proliferations, were found. These consisted of a single focus of plump mesothelial cells overlying an area of thick stroma. Often these foci included short, non-branched papillary projections above the surface of adjacent normal mesothelium. Twelve of the 16 lesions occurred in methyl(acetoxymethyl)nitrosamine-treated rats.
给78只5周龄雄性大鼠腹腔内单次注射甲基（乙酰氧基甲基）亚硝胺（13毫克/千克体重），诱发了25例间皮瘤；在67只对照大鼠中发现了2例间皮瘤。所有间皮瘤均起源于睾丸周围间皮，具有典型的显微镜下外观，即分支状乳头样叶，有胶原核心，被一层或多层丰满的肿瘤细胞覆盖。肿瘤细胞的细胞质中含有对胶体铁染色呈阳性反应且对透明质酸酶不稳定的物质。除了明显的间皮瘤外，还发现了16个病变，我们称之为非典型间皮增生。这些病变由覆盖在厚基质区域上的单个丰满间皮细胞灶组成。这些病灶通常包括在相邻正常间皮表面上方的短的、无分支的乳头状突起。16个病变中有12个发生在甲基（乙酰氧基甲基）亚硝胺处理的大鼠中。
