Christie W B, Newcomb R, Rowson L E
Vet Rec. 1979 Mar 31;104(13):281-3. doi: 10.1136/vr.104.13.281.
Fourteen heifers were superovulated five to 10 times using pregnant mare serum gonadotrophin (PMSG) and prostaglandin in a standardised regime which resulted in a mean interval of 42.1 days (SEM +/- 0.97) between treatments. There was a significant (P less than 0.02) fall in the mean ovulation rate at the second treatment when compared with the first but no significant difference between mean ovulation rate at the second and subsequent treatments. No significant difference between treatments in mean egg recovery rate or in the percentage of normal eggs recovered was demonstrated. Four heifers treated 10 times continued to respond with multiple ovulations on most occasions. At the end of the experiment 13 of the 14 heifers were inseminated and 12 conceived, requiring a mean of 1.67 services per pregnancy.
使用孕马血清促性腺激素(PMSG)和前列腺素,按照标准化方案对14头小母牛进行了5至10次超数排卵处理,两次处理之间的平均间隔时间为42.1天(标准误±0.97)。与第一次处理相比,第二次处理时平均排卵率显著下降(P<0.02),但第二次及后续处理的平均排卵率之间无显著差异。各处理在平均采卵率或回收的正常卵子百分比方面无显著差异。4头接受10次处理的小母牛在大多数情况下仍能多次排卵。实验结束时,14头小母牛中有13头进行了授精,12头受孕,每次怀孕平均需要1.67次输精。