变形链球菌已知血清型抗血清对葡糖基转移酶活性的抑制作用。
Inhibition of glucosyltransferase activity by antisera to known serotypes of Streptococcus mutans.
作者信息
Evans R T, Genco R J
出版信息
Infect Immun. 1973 Feb;7(2):237-41. doi: 10.1128/iai.7.2.237-241.1973.
Abstract
Using a recently developed assay for glucosyltransferase activity based on (14)C-glucose incorporation into an alcohol-insoluble polysaccharide, we were able to study inhibition by antibody of this enzyme activity. Rabbit antibody was relatively specific for the strain of Streptoccus mutans from which the enzyme was obtained. Absorption studies showed that neither removal of antibodies directed to dextran nor absorption with intact bacteria offset the enzyme-inhibitory capacity of these sera, whereas absorption with partially purified enzyme did result in removal of the inhibitory capacity.
摘要
利用最近开发的一种基于将（14）C - 葡萄糖掺入醇不溶性多糖来检测葡糖基转移酶活性的测定方法，我们能够研究抗体对该酶活性的抑制作用。兔抗体对从中获得该酶的变形链球菌菌株具有相对特异性。吸收研究表明，去除针对葡聚糖的抗体或用完整细菌进行吸收都不能抵消这些血清的酶抑制能力，而用部分纯化的酶进行吸收确实会导致抑制能力的消除。
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Inhibition of glucosyltransferase activity by antisera to known serotypes of Streptococcus mutans.变形链球菌已知血清型抗血清对葡糖基转移酶活性的抑制作用。
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2
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Arch Oral Biol. 1971 Aug;16(8):971-5. doi: 10.1016/0003-9969(71)90186-5.
3
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4
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5
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