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[鉴定病毒核蛋白组成中参与与DNA相互作用的氨基酸]

[Identification of the amino acids participating in interaction with DNA in the makeup of a viral nucleoprotein].

作者信息

Skliadneva V B, Chekanovskaia L A, Tikhonenko T I

出版信息

Vopr Med Khim. 1979 Jul-Aug;25(4):436-9.

PMID:473684
Abstract

A method is described for identification of amino acids participating in covalent DNA-protein cross-links formed in the course of modification of native CD phage by soldium bisulfite. The method is based on estimation of increased content of free amino acids, formed after treatment of the isolated cross-link products using equimolar mixture of O-methyl hydroxylamine and sodium bisulfite. Thin layer chromatography on the plates Fixion 50 x 8 was used for comparative semi-quantitative analysis of amino acids.

摘要

描述了一种鉴定参与由亚硫酸氢钠修饰天然CD噬菌体过程中形成的共价DNA-蛋白质交联的氨基酸的方法。该方法基于对使用O-甲基羟胺和亚硫酸氢钠等摩尔混合物处理分离的交联产物后形成的游离氨基酸含量增加的估计。使用在Fixion 50×8板上的薄层色谱法对氨基酸进行比较半定量分析。

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