一种从牛血浆中同时分离因子X和凝血酶原的方法。
A method for the simultaneous isolation of factor X and prothrombin from bovine plasma.
作者信息
Esnouf M P, Lloyd P H, Jesty J
出版信息
Biochem J. 1973 Apr;131(4):781-9. doi: 10.1042/bj1310781.
Abstract
- A method is described for the simultaneous isolation of both Factor X and prothrombin from bovine plasma. The proteins are adsorbed on and eluted from barium sulphate and chromatographed on DEAE-Sephadex and are finally purified by rechromatography on DEAE-Sephadex. 2. The proteins can be purified in 48h from the collection of the blood and the method can be used to process large volumes of plasma. 3. The prothrombin has a molecular weight of 70300; the Factor X, on the other hand, is polydisperse, with most of the protein (86%) having a molecular weight of 56000.
摘要
- 本文描述了一种从牛血浆中同时分离因子X和凝血酶原的方法。这些蛋白质先吸附于硫酸钡上，然后洗脱，接着在二乙氨基乙基葡聚糖凝胶（DEAE - Sephadex）上进行色谱分离，最后通过在DEAE - Sephadex上再次色谱分离进行纯化。2. 从采血开始，这些蛋白质可在48小时内得到纯化，该方法可用于处理大量血浆。3. 凝血酶原的分子量为70300；另一方面，因子X具有多分散性，大部分蛋白质（86%）的分子量为56000。
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