Pees H W
Klin Wochenschr. 1975 Jan;53(1):1-6. doi: 10.1007/BF01466850.
The cytotoxic action of lymphocytes on cancer cells in vitro indicates sensitization of the patient against his own tumor. The technical difficulities of this test and possibilities of standardization and simplifying the procedure are discussed. Critical steps are isolation of lymphocytes and culturing target cells without loosing their specific antigenic structure. The need for specificity controls both for lymphocytes and tumor cells is emphasized. Labelling tumor cells with isotopes represents a major improvement in evaluating the result. The role of thymus- and bone marrow-dependent lymphocytes as well as blocking factors in the serum of tumor patients can be analyzed in the cytotoxic assay. A better understanding of these mechanisms may facilitate a therapeutic approach by manipulating the interaction of tumor cells and host.
淋巴细胞在体外对癌细胞的细胞毒性作用表明患者对自身肿瘤产生了致敏反应。本文讨论了该检测方法的技术难点以及标准化和简化操作程序的可能性。关键步骤包括淋巴细胞的分离以及在不丧失其特定抗原结构的情况下培养靶细胞。强调了对淋巴细胞和肿瘤细胞进行特异性对照的必要性。用同位素标记肿瘤细胞是评估结果的一项重大改进。在细胞毒性试验中可以分析胸腺依赖性淋巴细胞和骨髓依赖性淋巴细胞以及肿瘤患者血清中阻断因子的作用。对这些机制的更好理解可能有助于通过操纵肿瘤细胞与宿主之间的相互作用来采取治疗方法。
