Muller R E, Johnston T C, Traish A M, Wotiz H H
Adv Exp Med Biol. 1979;117:401-21. doi: 10.1007/978-1-4757-6589-2_22.
A method for the preparation of viable uterine cell suspensions is described. Using this system the kinetics of estradiol uptake were studied in order to asses whether the steroid enters uterine cells by passive diffusion (1) or protein mediated diffusion (2). The data presented show that a) the rates of estradiol entry are nonsaturable; b) temperature dependence of uptake kinetics gives a linear Arrhenius plot; c) E2-6-CMO-BSA does not inhibit 3H-E2 uptake; d) uterine plasma membranes do not contain specific estrogen binding sites. Thus, estrogen uptake occures by passive diffusion.
本文描述了一种制备有活力的子宫细胞悬液的方法。利用该系统研究了雌二醇摄取的动力学，以评估该类固醇是通过被动扩散（1）还是蛋白质介导的扩散（2）进入子宫细胞。所呈现的数据表明：a）雌二醇进入的速率是不饱和的；b）摄取动力学的温度依赖性给出线性的阿伦尼乌斯图；c）E2-6-CMO-BSA不抑制3H-E2的摄取；d）子宫质膜不含有特异性雌激素结合位点。因此，雌激素的摄取是通过被动扩散发生的。
