Sulzer A J, Scholtyseck E, Callaway C, Smith M T, Huber T W
Am J Clin Pathol. 1979 Aug;72(2):225-9. doi: 10.1093/ajcp/72.2.225.
The analysis of fine structures by electron microscopic examination of ultrathin tissue sections permitted a diagnosis of toxoplasmosis in a fatal human case that would have gone undiagnosed by conventional methods. Examination of histologically prepared sections revealed organisms that were morphologically nondiagnostic. Fine-structural analysis showed the organisms to be 3 x 2 micrometer in size, to contain few micronemes, to contain as many as nine rhoptries, to possess an apical conoid, and to multiply by endodyogeny. The features corresponded to those observed in fine-structural analyses of Toxoplasma gondii grown in animals by Scholtyseck and Mehlhorn. Fine-structural analysis can be a valuable adjunct in the diagnosis of toxoplasmosis.
通过对超薄组织切片进行电子显微镜检查来分析精细结构，从而对一例致命的人类弓形虫病病例作出了诊断，而采用传统方法该病例可能无法确诊。对组织学制备切片的检查显示，这些生物体在形态上无法诊断。精细结构分析表明，这些生物体大小为3×2微米，含有少量微线体，多达九个棒状体，具有顶端锥体，并通过内二分裂进行繁殖。这些特征与Scholtyseck和Mehlhorn在动物体内培养的刚地弓形虫精细结构分析中观察到的特征一致。精细结构分析在弓形虫病的诊断中可能是一种有价值的辅助手段。
