Przybylski R J, Bullaro J C, MacBride R
Am J Physiol. 1979 Sep;237(3):C166-76. doi: 10.1152/ajpcell.1979.237.3.C166.
In primary cultures of chicken skeletal muscle, decreasing the [CO2] of the gaseous phase below 10(-3)% resulted in inhibition of cell proliferation and cytolysis. With 10(-3)% CO2-air, cell proliferation was slightly retarded and myotube formation was inhibited approximately 90% compared to cultures receiving 5% CO2-air. Changes in pH were not effective. Culture in low [CO2] resulted in the accumulation of lipoidal inclusions and unique cytoplasmic structures. Increasing time in culture with low [CO2] resulted in an increase in the length of G1 of the cell cycle. The inhibition was reversed by the addition of 5% CO2-air at any time in culture up to 2 wk with a minimum time of 3--6 h required. Lipoidal inclusions decreased in number and the unique cytoplasmic structures were absent. During the first 3 days in culture, myoblasts showing dependence on [CO2] for myotube formation increased in number, and the effect of elevated [CO2] on these cells was long lasting. The data suggest that some aspect of myoblast differentiation relating to cell recognition and fusion is affected by decreased [CO2].
在鸡骨骼肌原代培养物中，将气相中[CO₂]降低至10⁻³%以下会导致细胞增殖受抑制和细胞溶解。与在5% CO₂ -空气环境中培养的细胞相比，在10⁻³% CO₂ -空气环境下，细胞增殖略有延迟，肌管形成受到约90%的抑制。pH值的变化并无效果。在低[CO₂]环境中培养会导致脂类内含物和独特的细胞质结构积累。在低[CO₂]环境中培养时间的延长会导致细胞周期G1期长度增加。在培养长达2周的任何时间，加入5% CO₂ -空气均可逆转这种抑制作用，最短需要3 - 6小时。脂类内含物数量减少，独特的细胞质结构消失。在培养的前3天，显示出肌管形成依赖[CO₂]的成肌细胞数量增加，且[CO₂]升高对这些细胞的影响是持久的。数据表明，成肌细胞分化中与细胞识别和融合相关的某些方面受[CO₂]降低的影响。
