Wagner N, Degnan T J
J Clin Pathol. 1973 Sep;26(9):697-9. doi: 10.1136/jcp.26.9.697.
A new technique for detecting platelet antibodies was investigated. The serum from patients having platelet antibodies was added to normal group O compatible pooled platelet-rich plasma. Platelet aggregation induced by adrenaline and adenosine diphosphate (ADP) was significantly increased as compared to the identical platelet-rich plasma without serum. The platelet-rich plasma with serum added did not aggregate spontaneously. When an alpha-adrenergic blocking agent (tolazoline) was added, the primary wave of aggregation induced by adrenaline was equally reversed with platelet-rich plasma, with or without serum. Although platelet antibodies by themselves did not agglutinate platelets in our test system, it is probable that platelet antibodies interact with platelets and potentiate their ability to aggregate. It is suggested that the platelet aggregometer is a useful tool for the detection of platelet antibodies in vitro.
研究了一种检测血小板抗体的新技术。将患有血小板抗体患者的血清加入到与O型正常血兼容的富血小板混合血浆中。与不含血清的相同富血小板血浆相比，肾上腺素和二磷酸腺苷（ADP）诱导的血小板聚集显著增加。添加血清的富血小板血浆不会自发聚集。当加入α-肾上腺素能阻滞剂（妥拉唑啉）时，无论富血小板血浆是否含有血清，肾上腺素诱导的初级聚集波均同样被逆转。尽管在我们的测试系统中血小板抗体本身不会凝集血小板，但血小板抗体很可能与血小板相互作用并增强其聚集能力。提示血小板聚集仪是体外检测血小板抗体的有用工具。
