Fu E, Friedman L, Siegelman H W
Biochem J. 1979 Apr 1;179(1):1-6. doi: 10.1042/bj1790001.
The bile-pigment chromophores of C-phycoerythrin (phycoerythrobilin) and C-phycocyanin (phycocyanobilin) were cleaved from their respective proteins with boiling methanol or butan-1-ol. They were purified as dicarboxylic acids by preparative reverse-phase liquid chromatography. Each pigment existed in two principal forms, which were characterized by using 20 pmol samples by proton-transfer chemical-ionization mass spectroscopy. These two principal forms were isomeric species, and all had protonated parent molecular ions with m/e 587, corresponding to a molecular weight of 586.
用沸腾的甲醇或丁醇-1将C-藻红蛋白（藻红素）和C-藻蓝蛋白（藻蓝素）的胆汁色素发色团从各自的蛋白质上裂解下来。通过制备型反相液相色谱将它们纯化为二羧酸。每种色素以两种主要形式存在，通过质子转移化学电离质谱对20皮摩尔样品进行分析来表征这两种形式。这两种主要形式是异构体，且都有质荷比为587的质子化母分子离子，对应分子量为586。
