大鼠肝切片中胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的形成与分布。
The formation and distribution of bilirubin monoglucuronide and diglucuronide in rat liver slices.
作者信息
Campbell M T, Dutton G J
出版信息
Biochem J. 1979 Jun 1;179(3):473-7. doi: 10.1042/bj1790473.
Abstract
- Bilirubin conjugation in rat liver slices was reassessed by using analysis of ethyl anthranilate azopigments to estimate separately the formation of bilirubin mono- and di-glucuronides. 2. Conjugation in slices resembles the situation in vivo more closely than does microsomal conjugation, in that diglucuronide is formed in appreciable quantity. 3. Both bilirubin mono- and di-glucuronides were present in slices in approximately equal amounts, but the monoglucuronide was the major product found in the incubation medium. 4. These results are discussed in relation to recent theories on the relationship between bilirubin mono- and di-glucuronide formation in vivo.
摘要
- 通过使用邻氨基苯甲酸乙酯偶氮色素分析法分别估算胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的形成，对大鼠肝切片中的胆红素结合作用进行了重新评估。2. 切片中的结合作用比微粒体结合作用更接近体内情况，因为会形成相当数量的双葡萄糖醛酸酯。3. 胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯在切片中的含量大致相等，但单葡萄糖醛酸酯是孵育介质中发现的主要产物。4. 结合近期关于体内胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯形成之间关系的理论对这些结果进行了讨论。
相似文献
1
The formation and distribution of bilirubin monoglucuronide and diglucuronide in rat liver slices.大鼠肝切片中胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的形成与分布。
Biochem J. 1979 Jun 1;179(3):473-7. doi: 10.1042/bj1790473.
2
Mechanism of bilirubin diglucuronide formation in intact rats: bilirubin diglucuronide formation in vivo.完整大鼠体内胆红素二葡萄糖醛酸酯形成的机制：胆红素二葡萄糖醛酸酯的体内形成
J Clin Invest. 1980 Jun;65(6):1332-42. doi: 10.1172/JCI109797.
3
The formation of bilirubin diglucuronide by rat liver microsomal preparations.大鼠肝微粒体制剂生成胆红素二葡萄糖醛酸酯的过程。
Can J Biochem. 1980 Nov;58(11):1302-10. doi: 10.1139/o80-175.
4
Bilirubin mono- and di-glucuronide formation by purified rat liver microsomal bilirubin UDP-glucuronyltransferase.纯化的大鼠肝脏微粒体胆红素UDP-葡萄糖醛酸基转移酶催化胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的形成。
Biochem J. 1984 Oct 15;223(2):461-5. doi: 10.1042/bj2230461.
5
Bilirubin diglucuronide synthesis by a UDP-glucuronic acid-dependent enzyme system in rat liver microsomes.大鼠肝微粒体中由UDP-葡萄糖醛酸依赖性酶系统合成胆红素二葡萄糖醛酸酯。
Proc Natl Acad Sci U S A. 1979 Apr;76(4):2037-41. doi: 10.1073/pnas.76.4.2037.
6
The hepatic microsomal formation of bilirubin diglucuronide.胆红素二葡萄糖醛酸酯的肝微粒体形成。
J Biol Chem. 1983 Dec 25;258(24):15028-36.
7
Bilirubin diglucuronide formation in intact rats and in isolated Gunn rat liver.完整大鼠及分离的Gunn大鼠肝脏中胆红素二葡萄糖醛酸酯的形成
J Clin Invest. 1982 Mar;69(3):595-603. doi: 10.1172/jci110486.
8
Hepatic conversion of bilirubin monoglucuronide to diglucuronide in uridine diphosphate-glucuronyl transferase-deficient man and rat by bilirubin glucuronoside glucuronosyltransferase.胆红素葡糖苷酸葡糖醛酸基转移酶在尿苷二磷酸葡糖醛酸基转移酶缺陷的人和大鼠中，将单葡糖醛酸胆红素肝内转化为双葡糖醛酸胆红素。
J Clin Invest. 1978 Jul;62(1):191-6. doi: 10.1172/JCI109105.
9
Mechanism and subcellular site of bilirubin diglucuronide formation in rat liver.大鼠肝脏中胆红素二葡萄糖醛酸酯形成的机制及亚细胞定位
J Biol Chem. 1984 May 10;259(9):5500-6.
10
Bilirubin mono- and diglucuronide formation by human liver in vitro: assay by high-pressure liquid chromatography.人肝脏体外胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的形成：高压液相色谱法测定
Hepatology. 1981 Nov-Dec;1(6):622-7. doi: 10.1002/hep.1840010610.
引用本文的文献
1
Bilirubin glucuronidation by intact Gunn rat fibroblasts expressing bilirubin UDP-glucuronosyltransferase.表达胆红素UDP-葡萄糖醛酸基转移酶的完整Gunn大鼠成纤维细胞对胆红素的葡萄糖醛酸化作用。
Biochem J. 1996 Mar 1;314 ( Pt 2)(Pt 2):477-83. doi: 10.1042/bj3140477.
2
Mechanism of bilirubin diglucuronide formation in intact rats: bilirubin diglucuronide formation in vivo.完整大鼠体内胆红素二葡萄糖醛酸酯形成的机制：胆红素二葡萄糖醛酸酯的体内形成
J Clin Invest. 1980 Jun;65(6):1332-42. doi: 10.1172/JCI109797.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Biliary excretion of injected conjugated and unconjugated bilirubin by normal and Gunn rats.正常大鼠和冈恩大鼠对注射的结合胆红素和未结合胆红素的胆汁排泄。
Am J Physiol. 1961 May;200:1091-4. doi: 10.1152/ajplegacy.1961.200.5.1091.
3
Further studies on the nature and source of the conjugated bile pigments.关于结合胆汁色素的性质和来源的进一步研究。
Proc Soc Exp Biol Med. 1963 Apr;112:929-32. doi: 10.3181/00379727-112-28213.
4
The synthesis of bilirubin glucuronide in animal and human liver.胆红素葡萄糖醛酸酯在动物和人类肝脏中的合成。
Biochem J. 1958 Dec;70(4):705-12. doi: 10.1042/bj0700705.
5
Determination of bilirubin glucuronide and assay of glucuronyltransferase with bilirubin as acceptor.胆红素葡萄糖醛酸苷的测定及以胆红素为受体的葡萄糖醛酸转移酶的测定。
Biochem J. 1968 Apr;107(4):507-18. doi: 10.1042/bj1070507.
6
Determination of bilirubin UDP-glucuronyl transferase activity in needle-biopsy specimens of human liver.人肝穿刺活检标本中胆红素UDP-葡萄糖醛酸基转移酶活性的测定
Clin Chim Acta. 1970 Jul;29(1):27-35. doi: 10.1016/0009-8981(70)90216-0.
7
Metabolism of bilirubin by a clonal strain of rat hepatoma cells.大鼠肝癌细胞克隆株对胆红素的代谢
J Cell Biol. 1970 Dec;47(3):703-10. doi: 10.1083/jcb.47.3.703.
8
The formation of bilirubin mono- and diglucuronide by rat liver microsomal fractions.大鼠肝脏微粒体组分形成胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的过程。
Biochim Biophys Acta. 1972 Oct 25;279(3):544-53. doi: 10.1016/0304-4165(72)90176-6.
9
Recent advances in bilirubin formation, transport, metabolism and excretion.胆红素生成、转运、代谢及排泄的最新进展
Am J Med. 1970 Nov;49:576-89. doi: 10.1016/s0002-9343(70)80126-7.
10
Structures of bilirubin conjugates synthesized in vitro from bilirubin and uridine diphosphate glucuronic acid, uridine diphosphate glucose or uridine diphosphate xylose by preparations from rat liver.通过大鼠肝脏提取物，由胆红素与尿苷二磷酸葡萄糖醛酸、尿苷二磷酸葡萄糖或尿苷二磷酸木糖在体外合成的胆红素共轭物的结构。
Biochem J. 1972 Sep;129(3):635-44. doi: 10.1042/bj1290635.
Zotero 插件