Barza M, Bergeron M G, Brusch J, Weinstein L
Antimicrob Agents Chemother. 1973 Sep;4(3):337-42. doi: 10.1128/AAC.4.3.337.
Marked differences were noted among 14 antibiotics in their filterability through collodion (nitrated cellulose) membranes. The findings could not be attributed to differences in molecular weight or degree of serum protein binding, although the latter factor imposed an additional restraint on the passage of certain agents. Poorly filtered antibiotics appeared to be absorbed by the collodion. Some of these observations might be accounted for by the known ability of nitrated cellulose to interact with anions. However, the extreme differences in filterability among the various penicillins and the very restricted passage of certain cationic antibiotics appeared to be better explained by intrinsic differences in the diffusibility of these agents through water. Although differential filtration offers a means of assaying samples containing certain mixtures of antibiotics, the degree of separation exhibited by collodion membranes is not sufficient to make them useful for clinical purposes.
研究发现，14种抗生素透过火棉胶（硝化纤维素）膜的滤过性存在显著差异。这些结果不能归因于分子量或血清蛋白结合程度的差异，尽管后一因素对某些药物的通过施加了额外限制。滤过性差的抗生素似乎被火棉胶吸收。其中一些观察结果可能可以用硝化纤维素与阴离子相互作用的已知能力来解释。然而，各种青霉素之间滤过性的极端差异以及某些阳离子抗生素的极有限通过，似乎可以通过这些药物在水中扩散性的内在差异得到更好的解释。尽管差异过滤提供了一种检测含有某些抗生素混合物样本的方法，但火棉胶膜显示的分离程度不足以使其用于临床目的。
