Mustea I, Bara A
Br J Cancer. 1979 Aug;40(2):295-9. doi: 10.1038/bjc.1979.178.
The Crabtree effect can be observed when the O2 consumption of tumour cells or of mammalian cells grown in culture is measured in physiological medium containing glucose. The effect of 2 hypoxic cell radiosensitizers, misonidazole and NDPP, on the O2 consumption of Ehrlich ascites tumour cells was compared in media with and without glucose. A stimulatory effect on O2 consumption was found for 5--20mM misonidazole as well as for 0.5mM NDPP, both in media containing 10(-2)M glucose. Thus glucose induced a Crabtree effect in Ehrlich tumour cells, expressed as 38--45% inhibition of O2 consumption relative to that in the same medium without glucose. The stimulatory effect of misonidazole and NDPP on O2 utilization in medium with glucose undoubtedly appeared as a release from the Crabtree effect.
当在含有葡萄糖的生理培养基中测量肿瘤细胞或培养的哺乳动物细胞的耗氧量时，可观察到克拉布特里效应。在含葡萄糖和不含葡萄糖的培养基中，比较了两种乏氧细胞放射增敏剂米索硝唑和NDPP对艾氏腹水瘤细胞耗氧量的影响。在含有10⁻²M葡萄糖的培养基中，发现5 - 20mM米索硝唑以及0.5mM NDPP对耗氧量有刺激作用。因此，葡萄糖在艾氏肿瘤细胞中诱导了克拉布特里效应，相对于不含葡萄糖的相同培养基，耗氧量受到38 - 45%的抑制。米索硝唑和NDPP对含葡萄糖培养基中氧利用的刺激作用无疑表现为从克拉布特里效应中释放出来。
