一氧化二氮对人骨髓细胞周期的影响。

The effect of nitrous oxide on the cell cycle in human bone marrow.

作者信息

Cullen M H, Rees G M, Nancekievill D G, Amess J A

出版信息

Br J Haematol. 1979 Aug;42(4):527-34. doi: 10.1111/j.1365-2141.1979.tb01165.x.

DOI:10.1111/j.1365-2141.1979.tb01165.x

Abstract

The effect of 24 h exposure to nitrous oxide on the cell division cycle in human bone marrow has been studied in vivo using the technique of DNA flow microfluorimetry. All patients who received nitrous oxide showed a significant increase in the proportion of early S-phase cells with a decrease in late S, G2 and mitotic cells. These changes resemble those seen following the use of S-phase-specific cytotoxic drugs. Control patients showed no such effect. Parallel studies have suggested that interference with the function of vitamin B12 underlies this response. Nitrous oxide may provide a convenient method for studying the cell kinetic aspects of acute B12 deficiency and the possibility of using it to increase the therapeutic index of antitumour drugs is discussed.

摘要

采用DNA流式微量荧光测定技术，在体内研究了人体骨髓暴露于一氧化二氮24小时对细胞分裂周期的影响。所有接受一氧化二氮的患者，其早S期细胞比例显著增加，而晚S期、G2期和有丝分裂期细胞减少。这些变化类似于使用S期特异性细胞毒性药物后所见的变化。对照患者未出现此类效应。平行研究表明，这种反应的基础是对维生素B12功能的干扰。一氧化二氮可能为研究急性维生素B12缺乏的细胞动力学方面提供一种便捷方法，并且讨论了使用它来提高抗肿瘤药物治疗指数的可能性。