Gerritsen W J, Verkleij A J, Van Deenen L L
Biochim Biophys Acta. 1979 Jul 19;555(1):26-41. doi: 10.1016/0005-2736(79)90069-5.
Triton X-100 (in concentrations which did not cause a significant solubilization of membrane material) caused aggregation of the intramembrane particles of human erythrocyte ghosts. Ghosts from which the extrinsic proteins had been removed by alkali treatment showed a temperature-induced aggregation of the particles. With virtually no spectrin present, the particles in these stripped ghosts could still be aggregated by manipulations with ionic strength and pH, or by the addition of calcium. Recombinant vesicles were made from a Triton X-100 extract and a mixture of phospholipids with a composition which resembled that of the inner monolayer of erythrocyte membrane. In these recombinants the same manipulations with ionic strength and pH and the addition of calcium caused a rearrangement of the particles, resulting in the appearance of particle-free areas. In recombinants prepared from a Triton X-100 extract and egg phosphatidylcholine the lateral distribution of the particles was not altered by these manipulations. It is concluded that in the erythrocyte membrane the intramembrane particles can be aggregated by effects of external agents on lipid components. In this light the role of spectrin in stabilizing the membrane by interactions with lipids in the inner monolayer is discussed.
曲拉通X-100（浓度不会导致膜材料显著溶解）会引起人红细胞血影膜内颗粒的聚集。经碱处理去除外在蛋白后的血影显示出颗粒随温度诱导的聚集。在几乎不存在血影蛋白的情况下，这些去除了外在蛋白的血影中的颗粒仍可通过改变离子强度和pH值或添加钙来聚集。重组囊泡由曲拉通X-100提取物和一种磷脂混合物制成，该磷脂混合物的组成类似于红细胞膜内单层的组成。在这些重组体中，同样通过改变离子强度和pH值以及添加钙会导致颗粒重排，从而出现无颗粒区域。在由曲拉通X-100提取物和鸡蛋磷脂酰胆碱制备的重组体中，这些操作不会改变颗粒的侧向分布。得出的结论是，在红细胞膜中，膜内颗粒可因外部试剂对脂质成分的作用而聚集。据此讨论了血影蛋白通过与内单层脂质相互作用来稳定膜的作用。
