通过碳-13核磁共振和溶血磷脂酶测定溶血磷脂酰胆碱在肌浆网膜中的横向分布及移动
Transverse distribution and movement of lysophosphatidylcholine in sarcoplasmic reticulum membranes as determined by 13C NMR and lysophospholipase.
作者信息
van den Besselaar A M, de Kruijff B, van den Bosch H, van Deenen L L
出版信息
Biochim Biophys Acta. 1979 Aug 7;555(2):193-9. doi: 10.1016/0005-2736(79)90159-7.
PMID:476101
Abstract
- The transverse distribution of 1-palmitoyl-sn-glycero-3-phospho-N-[Me-13C]-choline in vitro incorporated in sarcoplasmic reticulum has been measured by means of 13C NMR and DyCl3 as an impermeable shift reagent. 2. Lysophosphatidylcholine added to the membranes equilibrates within 30 min at 20 degrees C between outer and inner membrane leaflet so that 42% is located in the inner leaflet. 3. Lysophosphatidylcholine diffuses back from the inner leaflet to the outer upon lysophospholipase action on the outer lysophosphatidylcholine pool.
摘要
- 借助碳-13核磁共振以及作为非渗透性位移试剂的三氯化镝，测定了体外掺入肌浆网的1-棕榈酰-sn-甘油-3-磷酸-N-[甲基-碳-13]-胆碱的横向分布。2. 添加到膜中的溶血磷脂酰胆碱在20摄氏度下30分钟内可在外膜小叶和内膜小叶之间达到平衡，使得42%位于内膜小叶中。3. 当溶血磷脂酶作用于外溶血磷脂酰胆碱池时，溶血磷脂酰胆碱从内膜小叶扩散回外膜小叶。
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