Braunschweiger P G, Schiffer L M
Cancer Res. 1979 Oct;39(10):3812-5.
The effect of methylprednisolone (MP) on the cell kinetic response to cyclophosphamide (CP) and Adriamycin (ADR) in C3H/HeJ spontaneous mammary tumors and hematopoietic tissue was investigated. The [3H]deoxythymidine labelingg index, the primer-dependent DNA polymerase labeling index (an estimate of tumor growth fraction), and the mitotic index were determined at various intervals after treatment. Treatment consisted of CP (200 mg/kg) on Day 0 plus ADR (2 mg/kg) on Day 1 with or without MP every 12 hr for 9 doses beginning on Day 2. In tumors treated with CP and ADR alone, changes in the kinetic parameters suggested proliferative recovery between Days 3 and 4 which coincided with bone marrow recovery. In tumors treated with CP, ADR, and MP, although the timing of the hematopoietic recovery was not affected by MP, the overshoot of the [3H]deoxythymidine labelin index on Days 3 and 4 was abolished. Proliferative recovery in the tumor was delayed until after cessation of MP treatments. Cell kinetic changes in the tumor after CP, ADR, and MP were used to design effective sequential chemotherapy which obviated the hematopoietic toxicity associated with sequential therapy designed from cell kinetic changes after CP and ADR alone.
研究了甲基强的松龙（MP）对C3H/HeJ自发性乳腺肿瘤及造血组织中细胞对环磷酰胺（CP）和阿霉素（ADR）的动力学反应的影响。在治疗后的不同时间间隔测定[3H]脱氧胸苷标记指数、引物依赖性DNA聚合酶标记指数（肿瘤生长分数的估计值）和有丝分裂指数。治疗方案为第0天给予CP（200mg/kg），第1天给予ADR（2mg/kg），从第2天开始，每12小时给予或不给予MP，共9剂。在单独用CP和ADR治疗的肿瘤中，动力学参数的变化提示在第3天和第4天之间有增殖恢复，这与骨髓恢复相一致。在用CP、ADR和MP治疗的肿瘤中，虽然造血恢复的时间不受MP的影响，但第3天和第4天[3H]脱氧胸苷标记指数的过冲现象被消除。肿瘤中的增殖恢复延迟到MP治疗停止之后。利用CP、ADR和MP治疗后肿瘤中的细胞动力学变化来设计有效的序贯化疗方案，从而避免了仅根据CP和ADR治疗后的细胞动力学变化设计的序贯疗法所带来的造血毒性。
