Stich H F, Wei L, Whiting R F
Cancer Res. 1979 Oct;39(10):4145-51.
Freshly prepared ascorbate inhibited mitosis and induced chromosome aberrations in cultured Chinese hamster ovary cells. Cu(II) and Mn(II) (10(-4) or 10(-5) M) enhanced both actions. Fe(II) and Fe(III) (10(-4) or 10(-5) M) reduced or abolished the mitosis-inhibiting action of ascorbate. At 10(-4) M, Fe(II) and Fe(III) strongly enhanced the chromosome-damaging capacity of ascorbate. Up to 100% of all examined metaphase plates had multiple chromosome exchanges or breaks. Since the cytostatic and clastogenic effect of ascorbate of H2O2 to induce chromosome aberrations was examined. H2O2 and a H2O2: Fe(II) mixture (Fenton reagent) induced chromosome breaks and exchanges but to a lesser degree than did ascorbate: Cu(II), Mn(II), Fe(II), or Fe(III) mixtures. Whether the strong chromosome damaging capacity of ascorbate plus transition metals as seen in the in vitro test system poses a health hazard only properly designed in vivo studies can reveal.
新制备的抗坏血酸抑制培养的中国仓鼠卵巢细胞的有丝分裂并诱导染色体畸变。铜（II）和锰（II）（10⁻⁴或10⁻⁵M）增强了这两种作用。亚铁（II）和铁（III）（10⁻⁴或10⁻⁵M）降低或消除了抗坏血酸的有丝分裂抑制作用。在10⁻⁴M时，亚铁（II）和铁（III）强烈增强了抗坏血酸的染色体损伤能力。所有检查的中期板中高达100%有多个染色体交换或断裂。由于研究了抗坏血酸、过氧化氢诱导染色体畸变的细胞抑制和致断裂作用。过氧化氢和过氧化氢：亚铁（II）混合物（芬顿试剂）诱导染色体断裂和交换，但程度低于抗坏血酸：铜（II）、锰（II）、亚铁（II）或铁（III）混合物。体外测试系统中所见的抗坏血酸加过渡金属的强大染色体损伤能力是否构成健康危害，只有经过适当设计的体内研究才能揭示。
