Costa M, Nye J S, Sunderman F W, Allpass P R, Gondos B
Cancer Res. 1979 Sep;39(9):3591-7.
In vitro exposure of Syrian hamster fetal cells to nickel subsulfide (alpha Ni3S2) yielded positive colony assays for morphological transformation. A dose-response relationship was found between the concentration of alpha Ni3S2 and the incidence of morphological transformation. Exposures of alpha Ni3S2 induced morphological transformation at concentrations (0.1 or 1.0 microgram/ml of culture medium) which did not impair cell plating efficiency. Nickel monosulfide (NiS) did not induce morphological transformation of Syrian hamster fetal cells under the same conditions. Clones of alpha Ni3S2-transformed cells were able to grow in soft agar medium and demonstrated increased basal and induced activities of ornithine decarboxylase. Undifferentiated sarcomas developed in 26 of 27 nude mice at the site of s.c. injection of clones of alpha Ni3S2-transformed cells. No tumors developed in 19 control nude mice which were given s.c. injections of nontransformed Syrian hamster fetal cells which had not been exposed to alpha Ni3S2. This study demonstrates that fetal cells which undergo transformation following exposure to alpha Ni3S2 are capable of producing malignant tumors in nude mice.
叙利亚仓鼠胎儿细胞在体外暴露于硫化亚镍（α-Ni3S2）后，菌落形态转化检测呈阳性。发现α-Ni3S2浓度与形态转化发生率之间存在剂量反应关系。在不损害细胞接种效率的浓度（0.1或1.0微克/毫升培养基）下，α-Ni3S2暴露诱导了形态转化。在相同条件下，硫化镍（NiS）未诱导叙利亚仓鼠胎儿细胞的形态转化。α-Ni3S2转化细胞的克隆能够在软琼脂培养基中生长，并显示鸟氨酸脱羧酶的基础活性和诱导活性增加。在27只裸鼠中，有26只在皮下注射α-Ni3S2转化细胞克隆的部位发生了未分化肉瘤。19只接受皮下注射未暴露于α-Ni3S2的未转化叙利亚仓鼠胎儿细胞的对照裸鼠未发生肿瘤。这项研究表明，暴露于α-Ni3S2后发生转化的胎儿细胞能够在裸鼠中产生恶性肿瘤。
