Anderson R E, Olson G B, Shonk C, Howarth J L, Wied C L, Bartels P H
Acta Cytol. 1975 Mar-Apr;19(2):126-33.
Computer-assisted morphometric analysis of murine lymphocytes obtained by thoracic duct cannulation demonstrates two populations of cells; the larger population (73 percent) appears to be thymus-derived and the remaining 27 percent is of bone marrow origin. Following exposure to varying amounts of x-radiation, morphologic alterations in both populations are evident. The smaller cell populations are evident. The smaller cell population exhibits some of these changes at lower dose levels than does the larger population. In addition, the character of the radiation-induced changes appears to be different for the two populations of lymphocytes. After 500 rad, the nuclei of the larger population appears unchanged; the nuclei of the population representing 27 percent of the cells have become enlarged and vacuolated and are thought to be edematous. After 2000 rad, the nuclei of the larger population appear pyknotic with coarsely clumped chromatin. In the examined set of cells, the smaller population could no longer be detected after 2000 rad. Such disparate responses to radiation-induced injury may correlate with known differences in immunologic function which serve to distinguish thymic-dependent and bone marrow-derived small lymphocytes.
通过胸导管插管获取的小鼠淋巴细胞的计算机辅助形态计量分析显示有两类细胞群体；较大的群体（73%）似乎源自胸腺，其余27%源自骨髓。在接受不同剂量的X射线照射后，两类群体的形态学改变都很明显。较小的细胞群体很明显。较小的细胞群体在较低剂量水平时就表现出其中一些变化，而较大的群体则不然。此外，辐射诱导的变化特征对于两类淋巴细胞群体似乎有所不同。500拉德照射后，较大群体的细胞核看起来没有变化；占细胞总数27%的群体的细胞核已变大且空泡化，被认为是水肿所致。2000拉德照射后，较大群体的细胞核呈现固缩，染色质粗糙聚集。在所检查的细胞组中，2000拉德照射后就不再能检测到较小的群体。这种对辐射诱导损伤的不同反应可能与已知的免疫功能差异相关，这些差异用于区分胸腺依赖性和骨髓源性小淋巴细胞。
