Anderson R E, Olson G B, Autry J R, Howarth J L, Troup G M, Bartels P H
J Immunol. 1977 Apr;118(4):1191-200.
Groups of 10-week-old female CBA/J mice were exposed in whole body fashion to 0,5,50, and 500 rads and sacrificed in serial fashion 1,3,5,7,9,15, and 30 days after irradiation for morphologic evaluation of thymus, spleen, lymph node, and Peyer's patch, and assessment of the relative numbers of thymus-derived (T) and bone marrow-derived (B) cells in these tissues. The absolute and relative numbers of recirculating T and B cells mobilizable by thoracic duct cannulation were also determined and compared with similar determinations with respect to peripheral blood lymphocytes. B cell depletion occurred more quickly and was more pronounced in spleen and lymph node than T cell depletion at all three exposure doses. Depletion of T and B cells was roughly equal in peripheral blood and thoracic duct lymph. When present, regeneration of the T cell component occurred more rapidly than did B cell restoration. The latter often was incomplete at the time of the final sacrifice (day 30). PHA-responsive and Con A-responsive cells also appeared to differ with respect to the kinetics of cell death after whole body irradiation.
将10周龄的雌性CBA/J小鼠分成几组，对其进行全身照射，照射剂量分别为0、5、50和500拉德，并在照射后1、3、5、7、9、15和30天依次处死，以对胸腺、脾脏、淋巴结和派伊尔结进行形态学评估，并评估这些组织中胸腺来源（T）细胞和骨髓来源（B）细胞的相对数量。还测定了通过胸导管插管可动员的循环T细胞和B细胞的绝对数量和相对数量，并与外周血淋巴细胞的类似测定结果进行比较。在所有三种照射剂量下，B细胞耗竭比T细胞耗竭发生得更快，并且在脾脏和淋巴结中更明显。外周血和胸导管淋巴液中T细胞和B细胞的耗竭大致相等。当T细胞成分出现再生时，其发生速度比B细胞恢复更快。在最后处死时（第30天），B细胞恢复通常不完全。全身照射后，PHA反应性细胞和Con A反应性细胞在细胞死亡动力学方面似乎也有所不同。
