人肝脏中酰基辅酶A-胆固醇酰基转移酶活性

Acyl-coenzyme A--cholesterol acyltransferase activity in human liver.

Balasubramaniam S, Mitropoulos K A, Myant N B, Mancini M, Postiglione A

Clin Sci (Lond). 1979 Apr;56(4):373-5. doi: 10.1042/cs0560373.

Abstract

In the presence of CoA and ATP, human liver microsomes catalyse the incorporation of [14C]oleate or [14C]cholesterol into cholesteryl oleate, thus demonstrating the presence of acyl-coenzyme A-cholesterol acyltransferase (cholesterol acyltransferase) in human liver. 2. The enzyme has properties similar to those of rat liver enzyme and with both the concentration of endogenous cholesterol in the microsomal fraction is adequate to support a constant initial rate of esterification. However, unlike the rat liver enzyme, the human cholesterol acyltransferase does not efficiently utilize added cholesterol as substrate. 3. The activity of cholesterol acyltransferase in human liver was 25% of that measured in rat liver under similar conditions of assay.

摘要

在辅酶A和三磷酸腺苷存在的情况下，人肝微粒体催化将[14C]油酸或[14C]胆固醇掺入胆固醇油酸酯中，从而证明人肝中存在酰基辅酶A - 胆固醇酰基转移酶（胆固醇酰基转移酶）。2. 该酶具有与大鼠肝酶相似的性质，并且微粒体部分中内源性胆固醇的浓度足以维持恒定的初始酯化速率。然而，与人肝酶不同，人胆固醇酰基转移酶不能有效地利用添加的胆固醇作为底物。3. 在相似的测定条件下，人肝中胆固醇酰基转移酶的活性是大鼠肝中测定活性的25%。