Slor H
Biochem J. 1973 Sep;136(1):83-7. doi: 10.1042/bj1360083.
Deoxyribonuclease II (DNAase II) in mammalian cells has generally been considered to be located in the lysosomes. Several recent studies have indicated that some DNAase II activity is present in purified nuclei; this, however, could have been due to some contamination of the nuclear fraction by lysosomes, or alternatively, it could have been caused by specific binding of lysosomal DNAase II to the nuclear fraction during isolation. Our previous studies have eliminated the possibility that lysosomal contamination was the cause of the presence of DNAase II in isolated nuclei. In this study I have purified (14)C-labelled lysosomal DNAase II and added it to cells during isolation of their nuclei. This study demonstrates that there is no specific binding of lysosomal DNAase II to the nuclear fraction and concludes that DNAase II activity observed in isolated nuclei represents an intrinsic activity that might be involved in nuclear DNA metabolism.
哺乳动物细胞中的脱氧核糖核酸酶II（DNAase II）通常被认为位于溶酶体中。最近的几项研究表明，在纯化的细胞核中存在一些DNAase II活性；然而，这可能是由于溶酶体对核部分的污染，或者，也可能是由于在分离过程中溶酶体DNAase II与核部分的特异性结合所致。我们之前的研究排除了溶酶体污染是分离细胞核中存在DNAase II的原因这一可能性。在本研究中，我纯化了用（14）C标记的溶酶体DNAase II，并在细胞核分离过程中将其添加到细胞中。这项研究表明，溶酶体DNAase II与核部分没有特异性结合，并得出结论，在分离的细胞核中观察到的DNAase II活性代表了一种可能参与核DNA代谢的内在活性。
