来自绿藻属和斜生栅藻的无细胞体系中的S-腺苷-L-甲硫氨酸-环阿屯醇甲基转移酶活性。
S-adenosyl-L-methionine-cycloartenol methyltransferase activity in cell-free systems from Trebouxia sp. and Scenedesmus obliquus.
作者信息
Wojciechowski Z A, Goad L J, Goodwin T W
出版信息
Biochem J. 1973 Oct;136(2):405-12. doi: 10.1042/bj1360405.
Abstract
- Homogenates prepared from Trebouxia sp. 213/3 and Scenedesmus obliquus exhibited S-adenosyl-l-methionine-cycloartenol methyltransferase activity. 2. The products of the reaction, with cycloartenol as the substrate, were 24-methylenecycloartanol and cyclolaudenol. 3. Optimal enzyme activity was found in homogenates prepared at pH7.6 and the transmethylase was distributed between the supernatant and microsomal fractions of the Trebouxia homogenate. 4. The relevance of these results is discussed in relation to C(28) and C(29) sterol production in the algae.
摘要
- 由绿藻门小球藻属213/3和斜生栅藻制备的匀浆表现出S-腺苷-L-甲硫氨酸-环阿屯醇甲基转移酶活性。2. 以环阿屯醇为底物时，反应产物为24-亚甲基环阿尔廷醇和环劳丹醇。3. 在pH7.6条件下制备的匀浆中发现了最佳酶活性，并且该甲基转移酶分布在小球藻匀浆的上清液和微粒体部分之间。4. 结合藻类中C(28)和C(29)甾醇的产生对这些结果的相关性进行了讨论。
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