对小鼠大脑听觉区域的一些神经化学研究。

Some neurochemical studies on auditory regions of mouse brain.

作者信息

Contreras N E, Bachelard H S

出版信息

Exp Brain Res. 1979 Aug 1;36(3):573-84. doi: 10.1007/BF00238524.

Abstract

The mouse brain auditory pathway has been dissected into five regions: geniculate bodies, posterior colliculi, superior olives, cochlear nuclei, and cochleas. The following analyses were made in these regions and in the auditory cortex: protein, glutamate, gamma-aminobutyrate, taurine, choline acetyltransferase, and glutamate decarboxylase. Taurine levels (nmol . mg of protein-) were highest in cortex (93) and geniculate bodies (60) and lowest in the cochlear nuclei (27) and cochleas (29). Concentrations of gamma-aminobutyrate (same units) were highest in the geniculate bodies (28), low in the superior olives and cochlear nuclei (9 to 10), and undetectable in the cochleas. The distribution of glutamate decarboxylase activity reflected that of gamma-aminobutyrate. The activities of choline acetyltransferase (nmol . of acetylcholine synthesized . h . -1 mg of protein-1) were highest in the superior olives (60) and low in the cochleas (3). These results are interpreted as biocyemical support for previous physiological and pharmacological identification of the olivo-cochlear bundle as cholinergic and the cochlear-nucleus neurones as non-cholinergic. The results also provide further evidence for a role of GABA in the posterior colliculi, but not in the cochleas.

摘要

小鼠脑听觉通路已被划分为五个区域

膝状体、后丘、上橄榄核、耳蜗核和耳蜗。对这些区域以及听觉皮层进行了以下分析：蛋白质、谷氨酸、γ-氨基丁酸、牛磺酸、胆碱乙酰转移酶和谷氨酸脱羧酶。牛磺酸水平（纳摩尔·毫克蛋白质⁻¹）在皮层（93）和膝状体（60）中最高，在耳蜗核（27）和耳蜗（29）中最低。γ-氨基丁酸浓度（相同单位）在膝状体中最高（28），在上橄榄核和耳蜗核中较低（9至10），在耳蜗中无法检测到。谷氨酸脱羧酶活性的分布反映了γ-氨基丁酸的分布。胆碱乙酰转移酶活性（纳摩尔·乙酰胆碱合成·小时⁻¹·毫克蛋白质⁻¹）在上橄榄核中最高（60），在耳蜗中较低（3）。这些结果被解释为对先前将橄榄耳蜗束鉴定为胆碱能、耳蜗核神经元鉴定为非胆碱能的生理和药理学研究的生化支持。这些结果还为γ-氨基丁酸在后丘而非耳蜗中的作用提供了进一步证据。