大鼠肝脏谷胱甘肽S-转移酶催化谷胱甘肽与1-萘基硫酸盐反应的机制。
The mechanism of the reaction between glutathione and 1-menaphthyl sulphate catalysed by a glutathione S-transferase from rat liver.
作者信息
Gillham B
出版信息
Biochem J. 1973 Dec;135(4):797-804. doi: 10.1042/bj1350797.
Abstract
- The glutathione S-transferase that catalyses the reaction of 1-menaphthyl (naphth-1-ylmethyl) sulphate with GSH was purified 76-fold from rat liver. 2. The properties of the purified enzyme were studied by gel filtration and isoelectric focusing. 3. The initial-velocity pattern in the absence of products and the product-inhibition pattern have been determined. These are consistent with an Ordered Bi Bi mechanism in which the GSH adds to the enzyme before 1-menaphthyl sulphate and the products are released in the order SO(4) (2-) followed by S-(1-menaphthyl)glutathione. 4. Dead-end-inhibition studies with p-aminobenzoic acid, which has been shown to be competitive with GSH and non-competitive with 1-menaphthyl sulphate, support the suggestion that an Ordered Bi Bi mechanism is operative. 5. Values were determined for some of the dissociation and Michaelis constants for the reaction of the substrates and products with the enzyme. 6. It appears that S-(1-menaphthyl)glutathione activates the enzyme when the concentration of GSH is saturating and that of 1-menaphthyl sulphate is low (of the order of its Michaelis constant).
摘要
- 催化1-萘基（萘-1-基甲基）硫酸酯与谷胱甘肽反应的谷胱甘肽S-转移酶从大鼠肝脏中纯化了76倍。2. 通过凝胶过滤和等电聚焦研究了纯化酶的性质。3. 测定了无产物时的初速度模式和产物抑制模式。这些与有序双底物双产物机制一致，即谷胱甘肽在1-萘基硫酸酯之前与酶结合，产物按硫酸根离子（SO(4) (2-)）然后是S-(1-萘基)谷胱甘肽的顺序释放。4. 对氨基苯甲酸的死端抑制研究表明，它与谷胱甘肽竞争，与1-萘基硫酸酯非竞争，支持了有序双底物双产物机制起作用的观点。5. 测定了一些底物和产物与酶反应的解离常数和米氏常数的值。6. 当谷胱甘肽浓度饱和而1-萘基硫酸酯浓度较低（约为其米氏常数）时，S-(1-萘基)谷胱甘肽似乎能激活该酶。
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本文引用的文献
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The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations.具有两种或更多种底物或产物的酶催化反应动力学。I. 命名法和速率方程。
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Biochemical studies of toxic agents. The metabolic formation of 1- and 2-menaphthylmercapturic acid.有毒物质的生化研究。1-和2-萘基巯基尿酸的代谢形成。
Biochem J. 1968 Apr;107(4):519-22. doi: 10.1042/bj1070519.
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Formation of mercapturic acids in rats after the administration of aralkyl esters.给予芳烷基酯后大鼠体内硫醚氨酸的形成。
Biochem J. 1970 Aug;118(5):765-71. doi: 10.1042/bj1180765.
6
The reaction of aralkyl sulphate esters with glutathione catalysed by rat liver preparations.大鼠肝脏制剂催化芳烷基硫酸酯与谷胱甘肽的反应。
Biochem J. 1971 Feb;121(4):667-72. doi: 10.1042/bj1210667.
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