单底物-单产物酶促反应的同位素交换稳态动力学。
The steady-state kinetics of isotope exchange for one substrate-one product enzymic reactions.
作者信息
Darvey I G
出版信息
Biochem J. 1973 Dec;135(4):861-6. doi: 10.1042/bj1350861.
Abstract
Steady-state kinetic equations for isotope exchange are derived for a number of one substrate-one product enzymic mechanisms in which two molecules of substrate or product can be combined with an enzyme molecule at the one time (e.g. allosteric mechanisms). The usual assumption, that the radioactive material is distributed among the substrate and product components according to a first-order law, is not valid. One can recognize whether isotope-exchange kinetics of an enzyme reaction follows first-order behaviour by using various initial concentrations of the labelled substance added to a mixture.
摘要
推导了多种单底物-单产物酶机制的同位素交换稳态动力学方程，其中底物或产物的两个分子可同时与一个酶分子结合（例如变构机制）。通常假设放射性物质根据一级定律分布在底物和产物组分之间，这是无效的。通过使用添加到混合物中的标记物质的各种初始浓度，可以识别酶反应的同位素交换动力学是否遵循一级行为。
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